Advanced Fertility Center of Chicago ivf

Freezing Spare Embryos in IVF Lab - Embryo Cryopreservation

Frozen embryo transfer cycle - FET

Sample Calendar for FET cycle

This page shows the older, traditional "slow freezing" method of embryo freezing. We are currently using vitrification - a new rapid freezing method that has resulted in better embryo survival and higher pregnancy success rates.

Cryopreservation area in IVF lab
The cryopreservation area of our lab
1: Liquid nitrogen supply tank
2: Backup embryo freezer
3: Main embryo freezer
4 & 5: Storage tanks for frozen embryos and sperm

Loading embryo freezer in IVF lab
The embryos are frozen in special straws in this device
- a controlled rate freezer
She is loading straws into the freezer

cryo cane
Straws that we freeze embryos in
From left to right in this image:
A nickel, 2 straws that we freeze embryos in, a cane that holds straws in the storage tank

cryo cane close
Here is a close-up of the same stuff
The 2 straws would also be labeled with names, etc. if they contained embryos

Seeding the straws in IVF lab
"Seeding" a straw at a critical temperature
This helps to avoid formation of intracellular ice
(ice formation can damage embryos)

Loading frozen embryos into the holding tank in IVF lab
In this picture, the cane with frozen embryos is being "plunged" into the storage tank
The tank contains liquid nitrogen at -196 degrees Centigrade (minus 320 degrees F)

Back to IVF lab tour


4920 N. Central Ave
Suite 2C

Chicago, IL 60630

Phone: (773) 794-1818
Fax: (773) 794-1819

30 Tower Court
Suite F
Gurnee, IL 60031

Phone: (847) 662-1818
Fax: (847) 662-3001

820 E. Terra Cotta Ave (Rte 176)
Suite 118
Crystal Lake, IL 60014

Phone: (815) 356-1818
Fax: (815) 356-1866



I am so grateful for Advanced Fertility Center. I just had my son Leland. I want to thank Dr. Catenacci and all of the nurses that helped us. Especially Ashley in the Crystal Lake office. You made this process easier by being supportive and positive. Thank you very much!


Dr. Sherban and his staff are the most amazing, compassionate, and supportive people. After almost 5 years of trying to conceive, going through numerous tests and meeting with another clinic closer to home, who told me my chances even with IVF were still very low, a good friend recommended advanced fertility center of Chicago. Best decision we ever made! We now have 2 amazing children who are now 5 and 3 years old! I cannot stress enough how amazing this staff is, they make you feel like family!


Our experience was amazing with Dr. Cantanucci and all of the nurses (especially Ashley). After ttc for 4 years and at 41 years of age, we became pregnant after 1 round of IVF, and had our beautiful boy this past October. Can't thank them enough!


2/7/15 our son was born all thanks to the assistance of Dr. Sherbahn and his staff. Everyone was so helpful and here for all our questions, worries, etc. They want this for you just as much as you want this for you! Handled with pure professionalism and care from the very start! Thank you advanced fertility!