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Assessment of effect of follicular fluid temperature at egg retrieval on blastocyst development, implantation and live birth rates

Research presentation by Dr. Richard Sherbahn at the 2010 Annual Meeting of the American Society for Reproductive Medicine

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Study title:Assessment of effect of follicular fluid temperature at egg retrieval on blastocyst development, implantation and live birth rates

Objective:To study the influence of follicular fluid temperature during the egg retrieval procedure on blastocyst development and implantation and live birth success rates

Design:Retrospective review of embryo development and implantation and live birth rates according to recorded follicular fluid temperatures at the egg retrieval.

  • Follicular fluid is recovered from the follicles in the ovaries when we aspirate the eggs

Materials and Methods:Our ability to maintain an ideal temperature for the oocytes during the time of the egg retrieval procedure was measured and recorded using thermocouple temperature sensors.

All (1659) IVF cycles with a female partner under age 43 that had follicular fluid temperature recorded between January 2002 and April 2009 were included in the study.

The cases were split into 3 groups for analysis:

  • The Ideal temperature group had readings between 36.4 and 36.9 degrees Centigrade
  • The Low group had readings below 36.4
  • The High group had temperatures above 36.9

IVF outcome parameters were evaluated including:

  • Day 5 blastocyst formation rate (% of fertilized embryos that were blastocysts on day 5
  • Implantation rates
  • Live birth rates
  • Statistical analysis utilized Student's t-test and chi-square statistics

Results: IVF cases with follicular fluid temperatures between 36.4 and 36.9 degrees Centigrade during egg retrieval had higher rates of blastocyst development, rates of embryo implantation and live birth success rates compared to cases that had lower or higher temperatures. Results shown in tables below.


Conclusions: Eggs are sensitive to temperature fluctuations. Maintaining egg temperatures in an ideal range during a retrieval procedure can improve embryo development and IVF success rates.

IVF Outcome by Follicular Fluid Temperatures - Ideal vs. Low

Means +/- SD

Ideal Temperature
Range 36.4 - 36.9

Age

32.8 +/- 4.2

Age range

20 - 42

Number ET

2.1 +/- 0.6

Blast development rate

2331/5684

41.0%

Implantation Rate

761/1898

40.1%

Live Birth Rate Per Retrieval

484/917

52.8%

Continued

IVF Outcome by Follicular Fluid Temperatures - Ideal vs. Low

Low Temperature
Range <36.4

P value

33.6 +/- 4.3

NS

21 - 42

 

2.1 +/- 0.7

NS

1408/4165

33.8%

P < 0.0001

482/1406

34.3%

P = 0.0006

293/665

44.1%

P = 0.0006

IVF Outcome by Follicular Fluid Temperatures - Ideal vs. Low

Means +/- SD

Ideal Temperature
Range 36.4 - 36.9

Low Temperature
Range <36.4

P value

Age

32.8 +/- 4.2

33.6 +/- 4.3

NS

Age range

20 - 42

21 - 42

 

Number ET

2.1 +/- 0.6

2.1 +/- 0.7

NS

Blast development rate

2331/5684

41.0%

1408/4165

33.8%

P < 0.0001

Implantation Rate

761/1898

40.1%

482/1406

34.3%

P = 0.0006

Live Birth Rate Per Retrieval

484/917

52.8%

293/665

44.1%

P = 0.0006

IVF Outcome by Follicular Fluid Temperatures - Ideal vs. High

Means +/- SD

Ideal Temperature
Range 36.4 - 36.9

Age

32.8 +/- 4.2

Age range

20 - 42

Number ET

2.1 +/- 0.6

Blast development rate

2331/5684

41.0%

Implantation Rate

761/1898

40.1%

Live Birth Rate Per Retrieval

484/917

52.8%

Continued

IVF Outcome by Follicular Fluid Temperatures - Ideal vs. High

High Temperature
Range >36.9

P value

33.4 +/- 4.5

NS

22 - 42

 

2.2 +/- 0.7

NS

139/457

30.4%

P < 0.0001

39/170

22.9%

P < 0.0001

29/77

37.7%

P = 0.015

IVF Outcome by Follicular Fluid Temperatures - Ideal vs. High

Means +/- SD

Ideal Temperature
Range 36.4 - 36.9

High Temperature
Range >36.9

P value

Age

32.8 +/- 4.2

33.4 +/- 4.5

NS

Age range

20 - 42

22 - 42

 

Number ET

2.1 +/- 0.6

2.2 +/- 0.7

NS

Blast development rate

2331/5684

41.0%

139/457

30.4%

P < 0.0001

Implantation Rate

761/1898

40.1%

39/170

22.9%

P < 0.0001

Live Birth Rate Per Retrieval

484/917

52.8%

29/77

37.7%

P = 0.015


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