Advanced Fertility Center of Chicago ivf

Video of ICSI procedure for in vitro fertilization - IVF Treat male factor infertility by fertilizing eggs in the IVF lab

Advanced Fertility Center of Chicago IVF with ICSI specialist clinic

Advanced Fertility Center of Chicago IVF with ICSI specialist clinic

What is ICSI - intracytoplasmic sperm injection?

Intracytoplasmic sperm injection (ICSI) is a very effective IVF technique for treating male infertility.

Intracytoplasmic means "into the cytoplasm" - the part of a cell that is inside the cell membrane.

  • In an egg there is an outer shell, called the zona pellucida, and under that the oolemma or cell membrane (see picture below).
  • Sperm are immobilized and picked up using a very small and sharp needle - and then injected into the cytoplasm of the egg.
  • In many IVF programs intracytoplasmic sperm injection results in fertilization of about 70% to 80% of the eggs injected with sperm.

Intracytoplasmic sperm injection sperm injection technique - video

Steps shown in video (also see image below):

  • Sperm is advanced to the tip of the needle
  • Egg is penetrated and needle advanced
  • Suction applied to break egg's outer membrane and mix sperm with egg's cytoplasm
  • Sperm and egg cytoplasm mixture injected into egg
  • Needle removed from egg
  • Egg released from holding pipette

ICSI video explained

Explanation for video above Holding pipette at left (H) ICSI needle at right Sperm head is seen in the needle Cumulus cells on outer shell (zona) came from the ovarian follicle Polar body of egg is at 12 o'clock (polar body shows egg is "mature")

4920 N. Central Ave
Suite 2C

Chicago, IL 60630

Phone: (773) 794-1818
Fax: (773) 794-1819

30 Tower Court
Suite F
Gurnee, IL 60031

Phone: (847) 662-1818
Fax: (847) 662-3001

820 E. Terra Cotta Ave (Rte 176)
Suite 118
Crystal Lake, IL 60014

Phone: (815) 356-1818
Fax: (815) 356-1866



I am so grateful for Advanced Fertility Center. I just had my son Leland. I want to thank Dr. Catenacci and all of the nurses that helped us. Especially Ashley in the Crystal Lake office. You made this process easier by being supportive and positive. Thank you very much!


Dr. Sherban and his staff are the most amazing, compassionate, and supportive people. After almost 5 years of trying to conceive, going through numerous tests and meeting with another clinic closer to home, who told me my chances even with IVF were still very low, a good friend recommended advanced fertility center of Chicago. Best decision we ever made! We now have 2 amazing children who are now 5 and 3 years old! I cannot stress enough how amazing this staff is, they make you feel like family!


Our experience was amazing with Dr. Cantanucci and all of the nurses (especially Ashley). After ttc for 4 years and at 41 years of age, we became pregnant after 1 round of IVF, and had our beautiful boy this past October. Can't thank them enough!


2/7/15 our son was born all thanks to the assistance of Dr. Sherbahn and his staff. Everyone was so helpful and here for all our questions, worries, etc. They want this for you just as much as you want this for you! Handled with pure professionalism and care from the very start! Thank you advanced fertility!