Advanced Fertility Center of Chicago ivf

Preparing Subcutaneous Injections for Fertility Treatment Shots

Preparing Subcutaneous Injections Using Vials

IVF hatching has been done using 3 general methods

  1. Remove syringe and needle (21 or 22G with 1 1/2 inch needle) from the wrapper, making sure needle is secure. Set aside.
  2. Remove plastic caps from prescribed # of vials of medication and diluent.
  3. Wipe tops of vials with alcohol to sterilize them. Don't touch the tops after they have been cleansed.
  4. Uncap needle by pulling cap straight out. Do not twist.
  5. Draw up 1 cc of air into syringe and inject into each powdered vial.
  6. Draw up 1cc of air and inject into the diluent vial. Tip the diluent vial and with needle in the fluid, withdraw fluid to the 1 cc mark. Remove needle from vial.
  7. Inject the diluent fluid into the vial of powder, gently aiming the fluid at the side of the vial.
  8. The powder will dissolve quickly-do not shake the vial. This will create bubbles.
  9. After the powder has dissolved, draw the reconstituted solution into the syringe.
  10. This can be done in 2 ways:
    • Leave the vial on the counter, tilting the vial at the end to withdraw the all of the solution.
    • After inserting the needle, raise the vial upside down to withdraw the medication.
  11. Slowly lower the needle as you withdraw all of the solution.

If you are instructed to reconstitute 2-4 vials, simply take your filled syringe, insert the needle into the rubber stopper of the second vial, and inject the reconstituted mixture into it. Withdraw the fluid as described previously. Repeat this procedure for each vial up to 4. If you are taking more than 4 vials at a time, they must be divided up into 2 shots.

After drawing up the last vial of medication, carefully recap the needle. Change the needle to the 25G 5/8 inch needle.

If the uncapped needle ever comes into contact with anything but the medication or diluent in the vial, such as your finger or countertop, immediately remove the needle and replace it with a new one.

Next, hold the syringe straight up and tap it so that any air bubbles rise to the top. To get rid of the bubbles, slowly press the plunger just a little, until a drop of liquid forms at the tip of the needle.

You are now ready to give your injection.

Details on giving the injection

Preparing Subcutaneous Injections Using Ampules

  1. Remove syringe and needle from wrapper (21 or 22 gauge 1 1/2 inch needle), making sure needle is secure. Set aside.
  2. Open the sodium chloride ampule by vertically holding the ampule base in one hand. With the other hand, grasp the top of the ampule between your thumb and index finger. With the dark dot facing away from you, snap the cap off in the direction away from you so the ampule breaks under the dot first. If done incorrectly, the ampule will shatter. Use an alcohol wipe or gauze pad under your fingers to protect them.
  3. Open the prescribed number of medication ampules in the same manner.
  4. Remove needle cover and draw up 1 cc ( or ml) of Sodium Chloride into the syringe.
  5. Slowly inject the Sodium Chloride into the open ampule of medication. If you inject the Sodium chloride too quickly, the medication will bubble up and you will have to wait until the bubbles subside. If you need to use 2 to 4 ampules of medication, inject the solution you have just drawn up from the first medications ampule into the second ampule and draw it up. Continue this for up to 4 ampules of medication. This is the maximum number of ampules that should be given in one injection.
  6. Carefully recap the needle after reconstitution is complete and change to a shorter needle (25gauge 5/8 inch) for administration.
  7. Remove air bubbles by holding the syringe straight up with needle pointing up and tap it so that any air bubbles rise to the top. To get rid of the air bubbles at the top, Slowly press the plunger just a little, until a drop of liquid forms at the tip of the needle.

You are now ready to give your injection.

Details on giving the injection

4920 N. Central Ave
Suite 2C

Chicago, IL 60630

Phone: (773) 794-1818
Fax: (773) 794-1819

30 Tower Court
Suite F
Gurnee, IL 60031

Phone: (847) 662-1818
Fax: (847) 662-3001

820 E. Terra Cotta Ave (Rte 176)
Suite 118
Crystal Lake, IL 60014

Phone: (815) 356-1818
Fax: (815) 356-1866



I am so grateful for Advanced Fertility Center. I just had my son Leland. I want to thank Dr. Catenacci and all of the nurses that helped us. Especially Ashley in the Crystal Lake office. You made this process easier by being supportive and positive. Thank you very much!


Dr. Sherban and his staff are the most amazing, compassionate, and supportive people. After almost 5 years of trying to conceive, going through numerous tests and meeting with another clinic closer to home, who told me my chances even with IVF were still very low, a good friend recommended advanced fertility center of Chicago. Best decision we ever made! We now have 2 amazing children who are now 5 and 3 years old! I cannot stress enough how amazing this staff is, they make you feel like family!


Our experience was amazing with Dr. Cantanucci and all of the nurses (especially Ashley). After ttc for 4 years and at 41 years of age, we became pregnant after 1 round of IVF, and had our beautiful boy this past October. Can't thank them enough!


2/7/15 our son was born all thanks to the assistance of Dr. Sherbahn and his staff. Everyone was so helpful and here for all our questions, worries, etc. They want this for you just as much as you want this for you! Handled with pure professionalism and care from the very start! Thank you advanced fertility!